What Is Bacteriostatic Water?
Bacteriostatic Water (BW) is sterile water containing 0.9% benzyl alcohol, which inhibits the growth of most bacteria. Unlike standard sterile water, it can be used multiple times from the same vial — making it the preferred choice for peptide reconstitution in research settings.
⚠️ Risk Notice: Bacteriostatic Water is intended for research use only. It is not approved for human or veterinary use. Improper handling may lead to contamination, degraded results, or safety hazards. Always follow your institution's laboratory safety protocols.
What You'll Need
- Lyophilized peptide vial
- Bacteriostatic Water — BacWater 3-Pack 10ml or BacWater 2-Pack 30ml (choose based on your volume needs)
- Alcohol swabs (70% isopropyl)
- Insulin syringe or reconstitution syringe
- Clean, flat surface
Step-by-Step Reconstitution Guide
Step 1 — Prepare Your Workspace
Work on a clean, disinfected surface. Wash your hands thoroughly or use gloves. Minimize air exposure to reduce contamination risk.
Step 2 — Swab All Stoppers
Use an alcohol swab to clean the rubber stopper of both the peptide vial and the BW vial. Allow to air dry for 10–15 seconds.
Step 3 — Draw the Bacteriostatic Water
Insert the syringe needle into the BW vial and draw the desired volume slowly. The amount depends on your target concentration — refer to your research protocol.
Step 4 — Inject Slowly Into the Peptide Vial
Angle the needle so the BW runs down the side of the vial, not directly onto the peptide powder. This prevents foaming and degradation.
Step 5 — Gently Swirl, Never Shake
Slowly swirl the vial in a circular motion until the powder is fully dissolved. Do not shake — agitation can break peptide bonds and reduce potency.
Step 6 — Inspect the Solution
The reconstituted solution should be clear and free of particles. If it appears cloudy or has visible debris, do not use it.
Step 7 — Label and Store Properly
Label the vial with the date of reconstitution and concentration. Store in a refrigerator (2–8°C) away from light. Most reconstituted peptides remain stable for 2–4 weeks when stored correctly.
Key Tips for Best Results
- Use the correct volume — more BW = lower concentration per unit
- Keep everything sterile — never touch needle tips or stopper surfaces
- Use within the recommended window — reconstituted peptides degrade over time
- One needle per draw — replace needles if they contact non-sterile surfaces
Why Choose Bac World Bacteriostatic Water?
Bac World's sterile multi-dose vials are manufactured to research-grade standards, ensuring consistent benzyl alcohol concentration, endotoxin control, and reliable multi-use performance — so your research results stay accurate and reproducible.
👉 Shop All BacWater Products — available in 10ml and 30ml multi-pack formats.
For research use only. Not intended for human or veterinary use.